The samples were analyzed for gut microbiota, fatty acids, antioxidant parameters, and the expression of mRNA and protein. At 4 h post-challenge, all pigs were slaughtered, and colonic samples were collected. After feeding with control or 0.2% tryptophan-supplemented diets for 35 days, pigs were intraperitoneally injected with LPS (100 μg/kg body weight) or saline. A total of 18 weaned castrated piglets were randomly divided into three treatments with 6 replicate per treatment, namely, (i) non-challenged control (CON) (ii) LPS-challenged control (LPS-CON) and (iii) LPS + 0.2% Trp (LPS-Trp). This study aimed to investigate the effect of Trp on intestinal barrier function, inflammation, antioxidant status, and microbiota in LPS-challenged piglets. However, there is no information about the effect of Trp on intestinal microbiota after lipopolysaccharide (LPS) challenge. Tryptophan (Trp) can modify the gut microbiota.
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